You may access the service at The Massive RT-PCR Primer Designer.
What this tool needs is your fasta file, which contains one or more nucleotide sequences that will be use as PCR template. Upload this file and the tool will generate the most ideal primer pairs suitable for quantitive Real-Time PCR (qRT-PCR). The primers will be supplied as a downloadable Excel file.
Notably, the amplicon size of these RT-PCR primers are spanning from 150 bp to 250 bp.
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